Table 1.
Plasmids used and constructed in this study
Plasmids | Description | Source of reference |
---|---|---|
pKTH10 | kan, amyQ; pUB110 | [33] |
pDAM | kan, amyM | AB Enzymes GmbH |
pHB201 2.4 | Cm, ermC, gfp | AB Enzymes GmbH |
pKVM2 | tetM; E. coli-Bacillus shuttle vector | AB Enzymes GmbH |
pBaSysBioII | spec, amp, gfpmut3 | [34] |
pDAPamy-gfp | kan; transcriptional P(amy)-gfp fusion | This study |
pDAPamy-gfp tet | tetM; transcriptional P(amy)-gfp fusion | This study |
pDAamyM-gfp | kan; out-of-frame translational amyM-gfp fusion | This study |