Fig. 10.

Colocalization of RAGE with HMGB1 and LAMP1 in MWCNT-treated cells. A549 cells were treated with 1 μg/mL MWCNT for 8 h, and then double immunofluorescent analysis was performed to examine the colocalization of RAGE with HMGB1 (a) and LAMP1, a lysosomal marker (b), as described in Methods. RAGE was stained with the mouse monoclonal antibody and Alexa 488-labeled goat anti-mouse IgG antibody. HMGB1 and LAMP were stained with the corresponding rabbit polyclonal antibodies and Alexa 594-labeled goat anti-rabbit IgG antibody. Hoechst, Hoechst 33258. Magnification, X400. Arrows indicate the colocalization of RAGE with HMGB1 or LAMP in the cytosol