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. Author manuscript; available in PMC: 2016 Mar 30.
Published in final edited form as: J Am Chem Soc. 2015 Dec 31;138(1):319–327. doi: 10.1021/jacs.5b10983

Figure 3.

Figure 3

A sample comprised of 13 DNA fragments ranging in size from 19 nt to 48 kbp is separated in the same 1.6 μm diameter capillary under both low ionic strength (20 mM EB buffer) and high salt conditions (20 mM EB + 25 mM NaCl). (a) Single molecule chromatograms of both separation conditions reveal that adding salt to the separation buffer induces relative mobility changes for small and large fragments. (b) Comparison of the effect of the added salt on ratiometric changes in relative mobility and packing density of the dsDNA fragments suggests a positive correlation between the two metrics.