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. 2015 Jul 27;63(11):842–853. doi: 10.1369/0022155415599039

Figure 6.

Figure 6.

Effects of ADMSC co-incubation on the apoptosis of NRK-52E cells exposed to high glucose (HG). (A) After growing to 90% confluence, NRK-52E cells were stimulated with normal glucose (NG, 5.5 mM glucose plus 24.5 mM mannitol) or HG (30 mM) for 12 hr. Then, the cells were co-incubated with ADMSCs for 24 hr. The apoptosis rates of NRK-52E cells in each group were measured by Annexin V-FITC/PI assay. Representative images are shown. (B) Real-time PCR analysis of Bax and Bcl-2 expression. (C) Western blot analysis of Bax and Bcl-2 expression. Data shown are the mean ± SD; n=3. $$p<0.01, compared with the NG+NRK-52E group; &p<0.05 and &&p<0.01, compared with the HG+NRK-52E group.