Figure 5.

Methods to enhance antibody reactivity/signals. Representative dual immunofluorescence images obtained with anti-CK14 (green, A–E) or anti-CD1a antibody (red, F–J) are shown after heating at 95°C for 45 min in various buffers in an attempt to enhance antibody reactivity/signals. Buffers used were: TE buffer, pH 9.0 (A, F); 10% citraconic acid, pH 6.0 (B, G); 10 mM Tris, pH 10.0 (C, H); 1 mM EDTA, pH 8.0 (D, I); and 10 mM citrate buffer, pH 6.0 (E, J). All tissues shown were stained with 0.3% Sudan Black B to reduce autofluorescence, but did not receive NH₃/EtOH or 3% H₂O₂ treatments. Scale, 0.5 mm.