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Methods to enhance antibody reactivity/signals. Representative immunofluorescence images obtained with anti-CK14 antibody (A–F) or anti-CD1a antibody (G–L) are shown without (A, G) and with additional steps to enhance antibody reactivity/signals: H2O2 in methanol prior to primary antibody (B, H): H2O2 in methanol prior to secondary antibody (C, I); pepsin (D, J); trypsin (E, K); or DTT/2-ME (F, L). See Materials & Methods for details of treatments. Scale, 0.5 mm.
