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. 2015 Nov 4;64(2):99–111. doi: 10.1369/0022155415617988

Figure 4.

Figure 4.

Western blot analysis of isolated normal human lung fibroblasts, untreated or transduced with CMV-GFP or CMV-Wnt7B adenovirus for 30 hr, then treated with or without 5 ng/ml TGF-β1 for 24 hr. Wnt7B overexpression (CMV-Wnt7B) and TGF-β1, separately, increased Wnt5A protein. This increase was enhanced by the combination of Wnt7B overexpression and TGF-β1. The positive staining reaction and appropriate molecular weight (~45 kDa) of the rhWnt5A-V5 band generated in pWnt5A-V5-transfected 293A cells and its absence in the pEGFP-transfected cells confirms the specificity of the CST 2530 anti-Wnt5A/B antibody. Vinculin was used as a loading control.