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. 2015 Nov 4;64(2):99–111. doi: 10.1369/0022155415617988

Figure 6.

Figure 6.

Western blot analysis of day 5-isolated normal human alveolar type 2 (hAT2) cells, untreated or transduced with CMV-LacZ or CMV-Wnt7B adenovirus (20 MOI) for 30 hr, then treated with or without 10 ng/ml TGF-β1 for 24 hr. TGF-β1 alone had no effect on Wnt5A expression but, when added to CMV-LacZ-transduced cells, Wnt5A expression increased significantly. Successful Wnt7B overexpression did not significantly increase Wnt5A expression, and did not add to the TGF-β1-stimulated increase. Transfected 293A rhWnt5A-V5-positive and -negative lysates are as in Figure 4. Tubulin was used as a loading control. The chart illustrates the results of band density quantitation, with standard deviation error bars shown. *p<0.05, **p<0.01.