Figure 8.

Co-localization of CS proteoglycans with 2B6 in the absence of chondroitinase ABC digestion. Cells were probed for the presence of 2B6 (A(i) and (ii)) and either perlecan (B(i)) or serglycin (B(ii)). Cells were then probed with anti-mouse Alexa Fluor 488 or anti-rabbit Alexa Fluor 594 secondary antibodies to detect the presence of 2B6 or the CSPGs, respectively. Merged channels demonstrate co-localization of 2B6 and perlecan (C(i) and D(i)), as well as 2B6 and serglycin (C(ii) and (D(ii)). Co-localization of 2B6 and serglycin/perlecan was quantified using the Manders co-localization coefficient (E). Scale (A, B, C) 50 µm; (D) 10 µm.