Table 1.
Details of the Primary Antibodies Utilized for Immunohistological Staining.
| Primary Antibody Epitope | Species | Isotype | Clone / Catalogue No. | Supplier | Dilution | Endoglyco-sidase Digestion* |
|---|---|---|---|---|---|---|
| Mast Cell Tryptase | Mouse | IgG | AA1 | Abcam | 2 µg/ml | – |
| Chondroitin-4 sulfate stub | Mouse | IgG | 2B6 | 1:500 | Yes | |
| Chondroitin-6 sulfate stub | Mouse | IgM | 3B3 | 1:500 | Yes | |
| Serglycin | Rabbit | Polyclonal | sc-292311 | Santa Cruz Biotechnology, Dallas, TX | 1:100 | – |
| Perlecan | Rabbit | Polyclonal | CCN-1 | ** | 1:1000 | – |
| Versican | Mouse | IgG | 5D5 | *** | 1:250 | Yes |
| Bikunin | Rabbit | Polyclonal | Ab43073 | Abcam, Cambridge, MA | 1:400 | – |
| Aggrecan | Mouse | IgG1 | 969D4D11 | Invitrogen, Carlsbad, CA | 0.2 µg/ml | Yes |
| CD86 | Rabbit | IgG | EP1158Y | Abcam | 1:200 | – |
| CD206 | Rabbit | Polyclonal | ab64693 | Abcam | 1:500 | – |
*
Digestion with chondroitinase ABC, 0.05 U/ml, pH 8.0, 0.1 M Tris-Acetate buffer.
**
Raised in house against immunopurified human coronary artery endothelial cell-derived perlecan (Lord et al. 2014; Whitelock et al. 1999).
***
A gift from Dr Firoz Rahemtulla, University of Alabama, Birmingham, AL, USA