Table 2.
Distribution of miR Gene Variants Among Patients With RM and Controls.
| Genotype | Patients, n = 200 (%) | Controls, n = 300 (%) | P Value | OR (95% CI) |
|---|---|---|---|---|
| miR-146aG > C (dbSNP ID rs2910164) | ||||
| Genotype frequency | ||||
| CC | 63 (31.5%) | 108 (36.0%) | 1 | – |
| CG (additive model) | 107 (53.5%) | 156 (52.0%) | .4813 | 1.17 (0.79-1.74) |
| GG (additive model) | 30 (15.0%) | 36 (12.0%) | .2379 | 1.42 (0.83-2.54) |
| GG + CG vs CC (dominant model) | .3361 | 1.22 (0.83-1.78) | ||
| GG vs CG + CC (recessive model) | .3473 | 1.29 (0.76-2.18) | ||
| Allele frequency | ||||
| C | 233 (58.3%) | 372 (62.0%) | .2356 | 0.85 (0.66-1.10) |
| G | 167 (41.7%) | 228 (38.0%) | .2356 | 1.16 (0.90-1.51) |
| miR-149T > C (dbSNP ID rs2292832) | ||||
| Genotype frequency | ||||
| TT | 128 (64.0%) | 207 (69.0%) | 1 | – |
| TC (additive model) | 62 (31.0%) | 84 (28.0%) | .4173 | 1.19 (0.80-1.77) |
| CC (additive model) | 10 (5.0%) | 9 (3.0%) | .2323 | 1.79 (0.71-4.54) |
| CC + TC vs TT (dominant model) | .2461 | 1.25 (0.85-1.82) | ||
| CC vs TC + TT (recessive model) | .3396 | 1.70 (0.67-4.26) | ||
| Allele frequency | ||||
| T | 318 (79.5%) | 498 (83.0%) | .1825 | 0.79 (0.57-1.09) |
| C | 82 (20.5%) | 102 (17.0%) | .1825 | 1.25 (0.91-1.73) |
| miR-196aT > C (dbSNP ID rs11614913) | ||||
| Genotype frequency | ||||
| TT | 65 (32.5%) | 104 (34.6%) | 1 | – |
| TC (additive model) | 95 (47.5%) | 158 (52.6%) | .9185 | 0.96 (0.64-1.43) |
| CC (additive model) | 40 (20.0%) | 38 (12.6%) | .0718 | 1.68 (0.97-2.89) |
| CC + TC vs TT (dominant model) | .6307 | 1.10 (0.75-1.61) | ||
| CC vs TC + TT (recessive model) | .0323a | 1.72 (1.06-2.80) | ||
| Allele frequency | ||||
| T | 225 (56.3%) | 366 (61.0%) | .1487 | 0.82 (0.63-1.06) |
| C | 175 (43.7%) | 234 (39.0%) | .1487 | 1.21 (0.94-1.57) |
| miR-499A > G (dbSNP ID rs3746444) | ||||
| Genotype frequency | ||||
| AA | 130 (65.0%) | 237 (79.0%) | 1 | – |
| AG (additive model) | 58 (29.0%) | 57 (19.0%) | .0045a | 1.85 (1.21-2.83) |
| GG (additive model) | 12 (6.0%) | 6 (3.0%) | .0109a | 3.64 (1.33-9.94) |
| GG + GA vs AA (dominant model) | .0006a | 2.02 (1.35-3.02) | ||
| GG vs GA + AA (recessive model) | .0258a | 3.12 (1.15-8.47) | ||
| Allele frequency | ||||
| A | 318 (79.5%) | 531 (88.5%) | .0001a | 0.50 (0.35-0.71) |
| G | 82 (20.5%) | 69 (11.5%) | .0001a | 1.98 (1.40-2.81) |
Abbreviations: CI, confidence interval; dbSNP, Single Nucleotide Polymorphism Database; NS, not significant; OR, odds ratio; RM, recurrent miscarriage.
aThe difference in frequencies between the case and control groups was analyzed for statistical significance at the 95% confidence interval using Fisher exact test with Bonferroni correction under additive, recessive, as well as dominant models of inheritance. Odds ratios were calculated and reported within the 95% confidence limits. Logistic regression analysis was performed with different genotypes as well as alleles as the independent factors, with the risk of RM being the dependent variable; additive model: comparing variant homozygous and heterozygous genotypes individually with wild-type homozygous genotypes; recessive model: comparing variant homozygous genotype with wild-type homozygous and heterozygous genotypes taken together; dominant model: variant homozygous and heterozygous genotypes taken together compared with wild-type homozygous genotype.