Protein expression of estrogen receptor β (ERβ) in the homogenate, cytosolic, and nuclear fractions of placentas. A, Representative Western blots for ERβ and β actin. B, No significant change was detected in expression of ERβ in the homogenate and cytosolic fractions on all dg; however, there was an increase in the expression in the nuclear fraction on 21 dg compared to that on 19 dg (*P < .05). Samples were run in duplicates; data are mean ± standard error of the mean (n = 4/group).