Structural organization of a rat placenta and negative and positive controls. A, A low-magnification photomicrograph of the placenta stained with Periodic acid-Schiff reaction showing basal (Bz) and labyrinth (Lz) zones. The location of decidual (Dc), glycogen (Gc), giant (Gic), and trophoblast (Tc) cells and villi are indicated, 40×. B, A negative control (without primary antibody) used simultaneously with confocal microscopy but stained for light microscopy showing no background labeling, 400×. C, Rat testis was used as a positive control for MTA1 showing labeled germ cells, 400×. Both negative and positive controls were counterstained with Mayer hematoxylin.