Colocalization of estrogen receptor (ER) α (TRITC) and ERβ (FITC) in placental cells. Confocal images showing expression of the ERs in different cells of the placenta. The nuclei are labeled with 4′,6-diamidino-2-phenylindole (DAPI; blue). Expression of ERα (red staining indicated by white arrows) and ERβ (green staining indicated by yellow arrows) is shown. Yellow to white labeling observed is due to colocalization of the proteins (mixture of green and red to variable degrees due to merging of images) and is indicated by open yellow arrows. These pictures show that ERα and ERβ are coexpressed in the cytoplasm and nucleus of decidual, glycogen, giant, and trophoblast cells on 16 dg, 19 dg, and 21 dg. Original magnification, 400×. (The color version of this article is available at http://rs.sagepub.com.)