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Sequence alignment of cpSRP43 CD1, CD2, CD3, Polycomb (Pc), and heterochromatin protein 1 (HP1). Green highlights conserved hydrophobic residues in CD2 that were mutated in this study. Red asterisks denote aromatic cage residues in canonical chromodomains. Black asterisks denote aromatic cage residues involved in 54M binding (23). Secondary structure information is labeled above the sequence highlighting β-sheet (β1, β2, β3) and α-helix (α1).
