Fig. S4.

Pairs of component cross-peaks in CD1 arise from the same amino acids. (Upper) Component cross-peaks of residues Ser92 (A) and Ala95 (B) in the TROSY spectrum of ²H, ¹⁵N, ¹³C-labeled CD1Ank-BH. (Lower) Strips from the HNCACB and HN(CO)CACB spectra of ¹⁵N, ¹³C, ²H-labeled CD1Ank-BH for the two component peaks of S92 and A95. For each residue, the two component peaks have connectivities to identical Cα and Cβ chemical shifts for residues i and i-1, indicating that they arise from the same amino acid. The red rectangles highlight the connectivities for the active state (Left) and inactive state (Right).