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Effects of 54M binding on the intensity of G264 (in BH). (A) Region of the TROSY spectrum of 2H, 15N-labeled cpSRP43 showing the effects of increasing concentrations of the 54M peptide on the G264 cross-peaks corresponding to unbound and 54M-bound cpSRP43 (labeled as peak 1 and peak 2, respectively). (B) Quantification of the relative intensities for the 54M-bound peak (peak 2) for G264. The line is a fit of the data to Eq. 1.
