Fig. S5.

Mutation in the E2F7 region showing sequence complementarity toward SNORD27. (A) Alignment of E2F7 pre-mRNA and SNORD27. The three mutated E2F7 sequences are shown below. (B) Effect of the mutations on E2F7 splicing patterns. HeLa cells were transfected with E2F7 mutated minigenes, and total RNA was analyzed by RT-PCR, similar to that shown in Fig. 2. The two samples represent replicates of the same condition. The quantification of splice isoforms ratio is shown in Right. (C) Alignment of mutated E2F7 pre-mRNA and SNORD27. The E2F7 minigene was mutated to compensate for the changes in the SNORD27 mutants. The sequence alignment shows complementarity between cotransfected pairs, where the mutated sequences are shown in red. (D) Effect of E2F7 compensatory mutations. HeLa cells were cotransfected with E2F7 mutated minigenes, with 3× molar excess of SNORD27 minigenes having compensatory mutations; RNA was analyzed by RT-PCR similar to B. M, molecular weight size marker.