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. 2016 Mar 7;113(12):3197–3202. doi: 10.1073/pnas.1523538113

Fig. 2.

Fig. 2.

iXCI is impaired in IVF female embryos. (A) Representative H3K27me3 immunostaining in cells isolated from embryonic (EM) and extraembryonic (EX) tissues of normal IVO (IVO N) and IVF (IVF N), as well as abnormal IVF (IVF ABN) female embryos at E7.5. (B) Percentage of H3K27me3-positive cells. Values with different letters are significantly different (P < 0.05). (C) Representative immunostaining for H3K27me3 (red) in the nuclei (DAPI) of IVO and IVF female blastocysts colabeled with CDX2 (green)-positive trophoblast cells. (Rightmost panels) Higher magnification of boxed regions. (D) The ratio of trophoblast cells classified by the number (0–2) of H3K27me3 domains. (E) Representative localization of Xist expression in the nuclei (DAPI) of IVO and IVF female morulae. (F) The ratio of blastomeres classified by the number (0–2) of Xist domains. (D and F) Each bar represents one female embryo. (G) Expression levels of Xist in female IVO and IVF embryos during preimplantation stage. Circles represent the relative expression value in each embryo. Horizontal gray lines represent the mean values. The number of embryos in each group is indicated. Early B, early blastocyst. *P < 0.05, **P < 0.01. (Scale bar, 50 μm.)