Fig. S2.

IVF embryos have lower survival rates after embryo transfer. (A) Survival rates of IVO and IVF embryos at E7.5, E10.5, and E19. The number (n) of embryos or fetuses in each group was indicated. (B) H&E staining of sagittal sections of IVO and IVF embryos with normal (N) or abnormal (ABN) morphology. ac, amniotic cavity; al, allantois; EM, embryo; EP, ectoplacental cone; epc, ectoplacental cone cavity; exc, exocoelomic cavity; ExE, extraembryonic ectoderm; F, female; M, male. Solid arrowhead indicates developmentally delayed embryos with normal morphology. Open arrowhead indicates a representative IVF female ABN E7.5 embryo that is missing extraembryonic tissues. (Scale bar, 100 μm.)