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iXCI status of IVF male embryos and expression patterns of X-linked genes in IVF female embryos. (A) Representative nuclear H3K27me3 immunostaining in IVO and IVF male blastocysts. Trophoblast cells were positively labeled with CDX2 staining. (B) Relative expression levels of representative X-linked genes to internal reference in pooled IVF and IVO female blastocysts. (C) Representative nuclear Xist noncoding RNA expression in IVO and IVF male morulae. (Scale bar, 50 μm.) *P < 0.05.
