Immunoblot quantifications of CPT1, PDH, and ACAD11 protein levels. Quantifications of CPT1, PDH-E1β, and ACAD11 relative to porin levels from three to four independent experiments; each comprises of a single mouse per time point at six time points throughout the day. (A) WT mice fed ad libitum. (B) PER1/2 null mice fed ad libitum. (C) Night-fed PER1/2 null mice. (D) WT mice fed with a high-fat diet for 3 d. Data are presented as fold change relative to the lowest value, with means ± SEMs of three to four different immunoblots. *P value < 0.05; **P value < 0.01; ***P value < 0.001.