Skip to main content
. 2016 Mar 7;113(12):3359–3364. doi: 10.1073/pnas.1524575113

Fig. 5.

Fig. 5.

Interaction of Htt with Spink3 inhibits trypsin activity. (A) Western blotting (Upper) and quantification (Lower) of Spink3 in 2-, 4-, and 8-mo-old ubiquitous KO and control mice. (n = 3 independent experiments, two-way ANOVA test; n.s. represents no significant difference, ***P < 0.001). (B) Immunoprecipitation of Htt with Spink3 from 2-, 4-, and 8-mo-old WT mouse pancreas. More Spink3 in 2-mo-old than 4- or 8-mo-old mouse pancreas was precipitated with Htt. Anti–α-tubulin and IgG IP were used as control. n = 3 independent experiments. One-way ANOVA test, *P < 0.05; **P < 0.01; ***P < 0.001. (C) Schematic structures (Upper) of Spink3 and Htt proteins, and Western blots (Lower) showing coimmunoprecipitation of transfected GFP, GFP-N-Spink3, or GFP-C-Spink3 and truncated HTT (tHTT) in HEK293 cells. IgG immunoprecipitation served as controls. (D) Relative activities of trypsin in the control and ubiquitous KO mouse pancreatic acinar cells that were transfected with full-length Htt (fHTT), N-terminal Htt (nHTT), and truncated Htt lacking N-terminal 169 amino acids (tHTT). n = 3 independent experiments, two-way ANOVA test, P < 0.05; **P < 0.01; ***P < 0.001.