Fig. S4.

RNAseq transcriptome analyses of T_MOG cells from T-MOG and T-/B-MOG mice and of naive T_MOG cells. Transcriptomes of effector T_MOG cells sorted from spleens of T-MOG and T-/B-MOG mice 9 d p.i. were compared and set in comparison with nonprimed T_MOG cells (n = 3). (A) Heat-map of the top 50 most differentially expressed genes between naive T_MOG cells and effector T_MOG cells from T-MOG and T-/B-MOG mice. Individual values of three different biological replicates from naive T_MOG cells and those from T-MOG and T-/B-MOG mice are shown. (B) Heat-map of 50 exemplary genes associated with relevant T-cell functions: i.e., activation and differentiation, cytokines and cytokine receptors, chemokine receptors, and cell locomotion genes. T_MOG cells from nonimmunized animals and those from T-MOG mice and T-/B-MOG mice were compared. (C) Gene expression levels of individual genes were normalized and are depicted as mean ± SEM. Genes are grouped according to function comprising genes involved in T-cell activation, cytokines, cytokine receptors, chemokine receptors, and adhesion molecules. The last group lists the four genes (out of more than 38,000 genes) that showed significantly differential expression in T_MOG cells from T-MOG mice and T-/B-MOG mice. Note that the differentially expressed Il-21 gene is grouped among the cytokine genes.