Fig. 4.

CheA clustering patterns in prototype interface 2 mutants. Plasmid pAV232 derivatives encoding CheA::mYFP/CheW in combination with an interface 2 lesion were induced at 0.3 µM sodium salicylate in strain UU1607 [∆(cheAW)], which contains a wild type complement of receptor proteins. Cells were imaged by fluorescence light microscopy. (A) Representative cell images showing the distribution of wild type and mutant CheA::mYFP proteins. Clustering contrast values were computed from the ratio of peak (highest) to body (mean of cells without the poles) intensities in each cell. (B) Distribution of clustering contrast scores for interface 2 mutants. Approximately 100 cells were analyzed for each mutant. (C) Normalized clustering scores for the interface 2 mutants in B. The averaged contrast values for each mutant were normalized to the wild type average.