. 2016 Mar 7;113(12):3335–3340. doi: 10.1073/pnas.1600216113

Table S1.

Plasmids used in this study

Plasmid	Properties/genotype of expression insert	Reference
Group 1	Derivatives of pBR322; confer ampicillin resistance; colE1 replication system; IPTG-inducible expression	40
pRR48	Vector	41
pRR53	tsr⁺	41
pCS53	tsr(S366C)	41
pPA803	yfp-cheR	42
pPA90	tsr[290-551]	43
Group 2	Derivatives of pACYC184; confer chloramphenicol resistance; p15A replication system; sodium salicylate-inducible expression	44, 45
pKG116	Vector	46
pPM25	cheA⁺ cheW⁺	This study
pGP55	cheA(M98L/C120S/C213S/C415S/A546C) cheA(S147ΩHA^b/A241ΩHA) cheW(E27C)	This study
pAV232	cheA(Δ[161-226]Ωmyfp) cheW⁺	This study
Group 3	Derivatives of pTrc99A; confer ampicillin resistance; colE1 replication system; IPTG-inducible expression	47
pVS88	cheY-yfp cheZ-cfp	28
pAV45	tar([QQQQ] Δ[528-553])-myfp	25

yfp, eyfp (enhanced YFP: S65G/V68L/S72A/T203Y) (48); HA, HA tag (YPYDVPDYA); myfp, eyfp + A206K (48); cfp, ecfp (enhanced CFP: F64L/S65T/Y66W/N146I/M153T/V163A) (48); tar [QQQQ], tar (E302Q/E491Q) (encodes Tar with a strongly ON-shifted output).