Table S2.
Expression level and chemotaxis performance of interface 2 mutant proteins
| Mutant protein | Expression level of mutant protein (fraction of WT)* | Chemotaxis on tryptone soft agar (fraction of WT)† |
| CheA-P5 | ||
| L545A | 0.85 | 0.45 |
| L545K | 0.75 | 0.40 |
| L545N | 0.75 | 0.50 |
| L545P | 0.85 | 0.40 |
| L545Q | 0.75 | 0.45 |
| L545R | 0.80 | 0.50 |
| L545S | 0.95 | 0.35 |
| V551A | 1.2 | 0.65 |
| V551D | 0.80 | 0.35 |
| V551E | 0.85 | 0.35 |
| V551G | 0.70 | 0.50 |
| V551L | ND | 0.95 |
| V551N | 0.80 | 0.40 |
| V551P | 0.75 | 0.35 |
| V551R | 0.85 | 0.40 |
| V551S | 0.70 | 0.50 |
| Y558D | 0.85 | 0.30 |
| Y558E | 0.95 | 0.30 |
| Y558G | 0.80 | 0.50 |
| Y558K | 0.80 | 0.30 |
| Y558N | 0.95 | 0.30 |
| Y558Q | 0.85 | 0.30 |
| Y558R | 0.95 | 0.30 |
| CheW | ||
| R117D | 0.75 | 0.45 |
| R117G | 0.75 | 0.35 |
| R117L | 0.75 | 0.95 |
| R117Q | 0.75 | 0.80 |
| E121A | 0.80 | 1.1 |
| E121R | 0.80 | 0.70 |
| F122K | 0.80 | 0.40 |
| F122R | 1.2 | 0.35 |
| F122S | 0.75 | 0.55 |
| F122Y | 0.75 | 0.85 |
| R117D/E121R/F122S (=X3) | 0.75 | 0.45 |
| Cross-linking reporters | ||
| CheA-A546C/CheW-wt | 1.0/0.95 | 1.1 |
| CheA-wt/CheW-E27C | 1.1/1.0 | 0.60 |
| CheA-A546C/CheW-E27C | 1.2/1.1 | 0.65 |
ND, not determined.
*
Expression of mutant proteins from plasmid derivatives was measured in strain UU2784 as described previously (16). Values <1.0 were rounded to the nearest 0.05, and values >1.0 were rounded to the nearest 0.1.
†
Mutant plasmids were tested in strain UU2683 for chemotaxis performance on tryptone semisolid agar. Values <1.0 were rounded to the nearest 0.05, and values >1.0 were rounded to the nearest 0.1.