Fig. 2.

Vascular organization within free-floating scaffolds upon inhibition of cell-generated forces. (A) HUVEC-GFP cells were embedded on a free-floating scaffold that was treated with an inhibitor (blebbistatin/Y27632) on day 3 of culture and later imaged on days 5 and 7. (Scale bars: 250 µm.) (B) Quantification of vessel quality determined by estimating the roundness parameter on days 5 and 7 postseeding. (C) Reformation of vascular networks on day 9, 2 d after removal of blebbistatin. (Scale bars: 250 µm.)