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. 2016 Mar 7;113(12):3215–3220. doi: 10.1073/pnas.1522273113

Fig. 3.

Fig. 3.

The orientation of vessel-like structures upon exposure to various mechanical stretching regimens. (A) Cyclic stretching applied on uniaxially fixated seeded constructs resulted in diagonal vessels whereas static stretching resulted in vertical vessels, and free-floating scaffolds resulted in randomly orientated vessels. Green, HUVEC-GFP cells. (Scale bars: 250 µm.) The presented images are the projection of about a 500-µm volume. (B) The orientation of cell actin fibers upon exposure to the mechanical forces described in A. Red, phalloidin staining; blue, DAPI. (C) Orientation analysis. Quantification of the average number of objects in 30–60° direction under cyclic stretching and control. (D) Orientation analysis. Quantification of the average number of objects in the stretching direction under static stretching and control.