Fig. S6.

Proangiogenic factor secretion under different mechanical conditions. Protein secretion from fibrin gels measured (A) 2 d or (B) 7 d postseeding. A comparison between free-floating gels (=1), uniaxially fixated gels, and gels attached to a plate. **P value < 0.01. This figure demonstrates the comparison between the different conditions of the fibrin gel in two time points. Attached gel resembles stronger forces than uniaxially fixated gel, all compared with the free-floating control. On day 2, we observed differences in the level of FGF, IL8, PDGF, Timp1, Timp2, and TNF. On day 7, we observed differences in ANG2, FGF, HFG, PDGF, and TNF. Similar to the Gelfoam construct, the level of PDGF increased under the induction of mechanical forces. In addition, the level of ANG2 increased with increasing forces on day 7. However, here, we couldn't identify changes in the level of VEGF.