Fig. S4.

Detection of 13-LOX in freeze-fractured chloroplasts and respective subfractions by electron microscopy. Detection of 13-LOX occurred in leaf tissues of plants that had been exposed to MeJA (A and C) or water (B) for 24 h. Whereas A and B show the plastid envelope regions of chloroplasts, C depicts an image of the thylakoid membrane fraction. Note that 13-LOX is detectable in outer envelope exoplasmic faces (OE-EF) (yellow circles) and inner envelope protoplasmic faces (IE-PF) (green boxes) (A and B) but not in the thylakoids (C). Immunodetection was made using anti-LOX plus 10-nm gold-conjugated secondary antibodies. Bars indicate scale markers.