Fig. 6.

Inactivating LMAN rescues aberrant song behavior in mHTT birds. (A) Schematic showing infusion of 5 mM muscimol with a microdialysis probe implanted into LMAN to inactivate LMAN activity. A representative song bout sung 1 h before (B) and 2 h after (C) infusion of muscimol in a mHTT bird is shown. Characters denote song syllables, and the square indicates the dominant motif. Increased sequence variability with irregular syllable insertion (red arrows in B) and repetition (red horizontal bar in B) decreased after muscimol infusion (C). Sequence entropy (D) and song bout duration (E) are shown as a function of time after infusion of saline (black) or muscimol (orange) in mHTT birds (n = 4 birds). Asterisks show significance (*P < 0.050), with a paired t test, after the significant main effect of condition was confirmed with two-way ANOVA [sequence entropy [F(1,58) = 10.41, P = 0.002; bout duration: F(1,58) = 11.05, P = 0.002] (see also Fig. S8).