Figure 10.

Targeted neurons exhibit partial overlap with the DR1 expressing population. (A) Co-injection of AAV-CamKII-hM3D(Gq) and Cre-dependent SUN2-myc into DR1-Cre mice demonstrated that the neurons targeted in this study only partially overlap with the DR1-expressing population. (A₁) SUN2 expression, demonstrating DR1 promoter activity; (A₂) mCherry expression, showing AAV-hM3D(Gq) expression; (A₃) Merged image, with example cells of interest identified. (B) A parallel experiment using AAV-CamKII-eYFP instead of AAV-CamKII-hM3D(Gq) showed similar results. (B₁) eYFP epifluoresence; (B₂) SUN2 expression; (B₃) Merged image. In (A,B), Filled arrowheads: DR1/SUN2 expression only; Open arrowheads: dual expression of DR1/SUN2 and eYFP/mCherry/DREADD expression; Arrows: eYFP/mCherry/DREADD expression only. In (C), arrows indicate examples of the expression of both SUN2-myc (C₁), tdTomato (C₂), and Merge (C₃) in a control experiment, in VGAT-Cre-tdTomato mice (Vong et al., 2011). No Sun2-myc single positive neurons were observed. Scale bars are 100 μm.