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. 2016 Feb 25;17(3):233. doi: 10.3390/ijms17030233

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cold stress and hormone-dependent expression of ROC1. (a) Fifteen-day-old wild-type seedlings were grown in a normal rice growth chamber (28 °C) and were moved into a low temperature growth chamber (4 °C), and then, the seedlings were sampled after 0, 1, 3, 6, 12 and 24 h. ROC1 expression levels were monitored with qRT-PCR; (b) Hormonal-regulation of ROC1 transcription. Fifteen-day-old seedlings were treated with 1 μM ABA, 1 μM NAA, 0.1 μM 2,4-epiBL, 1 μM GA and 1 μM ACC for 3 h. qRT-PCR was performed to analyze the expression patterns of ROC1 upon hormone treatment. The expression levels were normalized against that of Ubiquitin mRNA. Significant differences between NAA-treated and -untreated samples (control, CK) are shown (* p < 0.05).