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. 2016 Feb 25;17(3):233. doi: 10.3390/ijms17030233

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cold stress-mediated expressions of CBF1 and CBF3 in ROC1 mutants. (a) Fifteen-day-old wild-type and roc1 seedlings were grown in a normal rice growth chamber (28 °C) and were moved into a low temperature growth chamber (4 °C). The seedlings were sampled after 0, 1, 3, 6, 12, 24 and 48 h. The expression levels of CBF1 and CBF3 were detected by Northern blot analysis; (b) The levels of CBF1 and CBF3 were analyzed in wild-type and two ROC1 RNAi lines (Ri3 and Ri5) after 0, 1 and 3 h of cold treatment. Ethidium bromide (EtBr) staining of rRNA is shown as the loading control.