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. 2016 Feb 23;17(3):267. doi: 10.3390/ijms17030267

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ischemic preconditioning (IPC) treatment induced upregulation of TOPK in the rat myocardium after 30 min ischemia/3 h reperfusion. (A) Representative immunohistochemical staining of TOPK and p-TOPK in the ischemic area of left ventricular (LV) myocardial sections of rats subjected to sham operation, I/R, or IPC+I/R; (B) Detection of mRNA levels of TOPK by RT-PCR. GAPDH was used as the loading control; (C) Detection and quantitative analysis of TOPK and p-TOPK levels by western blotting. β-actin was used as the loading control. n = 6. * p < 0.05 compared with sham-operation group.

Ischemic preconditioning (IPC) treatment induced upregulation of TOPK in the rat myocardium after 30 min ischemia/3 h reperfusion. (A) Representative immunohistochemical staining of TOPK and p-TOPK in the ischemic area of left ventricular (LV) myocardial sections of rats subjected to sham operation, I/R, or IPC+I/R; (B) Detection of mRNA levels of TOPK by RT-PCR. GAPDH was used as the loading control; (C) Detection and quantitative analysis of TOPK and p-TOPK levels by western blotting. β-actin was used as the loading control. n = 6. * p < 0.05 compared with sham-operation group.