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Levels of ER stress-related proteins were upregulated in compressed tissues. The protein expression of GRP78, CHOP, and cleaved caspase-12 was determined by western blotting. (A) GAPDH was used as a protein loading control and for band density normalization; (B–D) The optical density of GRP78, CHOP, and cleaved caspase-12 protein levels were analyzed and plotted. Data are presented as the mean values ± SD. n = 8. * p < 0.05; ** p < 0.01 as compared with control group.
