Table 2.
Description of the antibodies used and their manufacturers, and of immunocytochemistry protocols and detection methods.
| Antibody | Vendor | Epitope Retrieval | Primary Antibody (Dilution; Incubation Time) | Method of Detection | Positive Control |
|---|---|---|---|---|---|
| anti-CD4 | Dako (Carpinteria, CA, USA) | PT-Link, High pH | RTU; 20 min | Mouse Linker/EnVision/DAB (Dako) | lymph node |
| anti-CD8 | Dako | PT-Link, High pH | RTU; 20 min | EnVision/DAB (Dako) | lymph node |
| anti-CD20 | Dako | PT-Link, High pH | RTU; 20 min | EnVision/DAB (Dako) | lymph node |
| anti-CD25 | Ventana Medical Systems, Inc. (Tucson, AZ, USA) | manufacturer’s protocol | RTU; manufacturer’s protocol | UltraView Universal DAB (Ventana) | lymph node |
| anti-FoxP3 | Abcam (Cambridge, UK) | PT-Link, Low pH (Dako) | 1:100; O/N 4 °C | EnVision/DAB (Dako) | lymph node |
O/N, overnight; DAB, 3,3′-diaminobenzidine; RTU, ready to use.