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. 2016 Feb 26;17(3):292. doi: 10.3390/ijms17030292

Table 1.

The sequences of the oligonucleotide primers for quantitative real-time polymerase chain reaction (qRT-PCR) assays.

Gene Primers Sequence Amplicon Size (bp) Accession Number
Col-I Forward: 5′-CCTGAGTCAGCAGATTGAGAACA-3′ 114 NM_007742
Reverse: 5′-CCAGTACTCTCCGCTCTTCCA-3′
OC Forward: 5′-CGCTCTGTCTCTCTGACCTC-3′ 91 NM_001037939
Reverse: 5′-CACTACCTTATTGCCCTCCTG-3′
OPN Forward: 5′-CTTTCACTCCAATCGTCCCTAC-3′ 99 NM_001204201
Reverse: 5′-CAGAAACCTGGAAACTCCTAGAC-3′
ALP Forward: 5′-GGGCATTGTGACTACCACTCG-3′ 103 NM_001287172
Reverse: 5′-CCTCTGGTGGCATCTCGTTAT-3′
Runx2 Forward: 5′-GACACTGCCACCTCTGACTT-3′ 115 NM_001145920
Reverse: 5′-GATGAAATGCTTGGGAACTG-3′
GAPDH Forward: 5′-CATCAAGAAGGTGGTGAAGC-3′ 198 NM_001289726
Reverse: 5′-CCTGTTGCTGTAGCCGTATT-3′