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. 2016 Mar 1;17(3):323. doi: 10.3390/ijms17030323

Figure 3.

Figure 3

Apigenin has no effect on LPS-induced splenocyte apoptosis. (A) H&E staining (top), TUNEL (middle) and immunohistochemistry (IHC) for active caspase-3 (bottom) in spleens from control, Api, LPS and Api + LPS mice at 24 h. In all pictures scale bars represent 10 μm, arrows indicate apoptotic cells; (B) Quantification of apoptotic cells as determined by H&E; (C) Number of apoptotic cells detected by TUNEL; (D) Number of apoptotic cells detected by staining with anti-active caspase-3 specific antibodies; (E) Percentage of apoptotic cells determined by Annexin V/Propidium Iodide (PI) staining using flow cytometry. Data represent the mean ± SEM (N = 5 mice for each condition; no statistical difference between LPS and Api + LPS).