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. 2016 Mar 1;17(3):323. doi: 10.3390/ijms17030323

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Apigenin reduces LPS-induced lung inflammatory chemokines. Bronchoalveolar lavage fluid (BALF) and whole lungs were obtained from control, Api, LPS and Api + LPS mice. (A,C) Macrophage inflammatory protein-2 (MIP-2) and keratinocyte chemoattractant (KC) protein expression was determined by ELISA in BALF; (B,D) MIP-2 and KC steady-state mRNA levels in whole lung were determined by quantitative RT-PCR. Values represent the means ± SEM (N = 6 mice for each biological condition and time point; * p < 0.05, compared to LPS-treated mice at the same time point). RCN, relative copy number.