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. 2016 Mar 11;17(3):359. doi: 10.3390/ijms17030359

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of lubricin immunostaining. (A,B) Lubricin immunohistochemistry specimen from control (A) and sham (B) cartilage (without ACLT) showed a very strong (ES = +++; IS = 4) immunostaining in chondrocytes from superficial and middle zone of rat femoral articular cartilage; (C) Lubricin immunohistochemistry specimen from moderate OA cartilage (with ACLT) showed a weak/absent (ES = +; IS = 1) immunostaining in chondrocytes from rat femoral articular cartilage (superficial, middle and deep zone); (D) The negative control treated with PBS without the primary antibody (lubricin) did not show immunostaining (ES = 0; IS = 0). (A–D) Magnifications ×20; Scale bars: 100 µm; (E) Immunohistochemical evaluation graph: percentage of lubricin positive cells out of the total number of cells counted in control groups and in the OA group. Results are presented as the mean ± SEM. ANOVA was used to evaluate the significance of the results. * p < 0.01, when compared to the control groups.

Evaluation of lubricin immunostaining. (A,B) Lubricin immunohistochemistry specimen from control (A) and sham (B) cartilage (without ACLT) showed a very strong (ES = +++; IS = 4) immunostaining in chondrocytes from superficial and middle zone of rat femoral articular cartilage; (C) Lubricin immunohistochemistry specimen from moderate OA cartilage (with ACLT) showed a weak/absent (ES = +; IS = 1) immunostaining in chondrocytes from rat femoral articular cartilage (superficial, middle and deep zone); (D) The negative control treated with PBS without the primary antibody (lubricin) did not show immunostaining (ES = 0; IS = 0). (A–D) Magnifications ×20; Scale bars: 100 µm; (E) Immunohistochemical evaluation graph: percentage of lubricin positive cells out of the total number of cells counted in control groups and in the OA group. Results are presented as the mean ± SEM. ANOVA was used to evaluate the significance of the results. * p < 0.01, when compared to the control groups.