Figure 2.

Mg²⁺ binding to the DNA 4WJ as measured by isothermal titration calorimetry. (a) Raw data and (b) normalized integration of the raw data for the titration of 0.85 mM Mg²⁺ into a 16 μM solution of DNA 4WJ; (c) Residuals depicted are generated between the actual data and the fit shown. Both solutions contained 5 μM EDTA, 5 mM Na⁺, and 10 mM Tris–HCl pH 7.4. Two titrations of 25 × 10-μL injections were performed and appended using the Concat32 software (MicroCal of Malvern Instruments Ltd., Worchestershire, UK).