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. 2016 Mar 11;17(3):371. doi: 10.3390/ijms17030371

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Titration of apo-γ-E_c-1 with Cu(I) ions followed by CD spectroscopy. (A) Evolution of CD spectra; (B) course of ellipticity values at or close to the wavelength of the maxima observed in the CD spectra; and (C) comparison of selected spectra from (A) with the respective spectra obtained during the titration of Zn₂γ-E_c-1 with Cu(I) ions (see Figure S7).