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. 2016 Mar 18;17(3):404. doi: 10.3390/ijms17030404

Figure 2.

Figure 2

FGA haloperidol increases the lysosomal pH. HepG2 cells were incubated with LDL. (30 µg/mL of cholesterol) for 12 h in the absence (control) or the presence of haloperidol (Hal) (5, 10 or 25 µM) and pH-sensing fluorochromes Alexa Fluor 488-dextran and pHrodo-dextran. (A) Sample confocal images of control and 10 µM haloperidol treated culture dishes showing emission reduction of pH sensitive pHrodo (in red) relative to pH insensitive Alexa Fluor probe (in green). Scale bar, 5 µm. (B) Standard curve to calculate endogenous lysosomal pH where values of the red:green fluorescence ratio corresponding to control and haloperidol at 10 µM are indicated. (C) Effect of increasing concentrations of haloperidol on lysosomal pH. Results are mean ± SEM (n = 3–15). Statistical comparisons shown are haloperidol versus control (**** p <0.0001).