Fig. 5.

NTF2 levels affect nuclear size in mammalian tissue culture cells. (A) HeLa and MRC-5 cells were transiently transfected with plasmids expressing mCherry or mCherry–NTF2. At 48 h after transfection, cells were fixed and stained with Hoechst 33342 to visualize the DNA (blue). Representative images are shown. (B) Nuclear cross-sectional areas were quantified from Hoechst-33342-stained cells that were identified as being transfected by mCherry expression. For each data point, the cross-sectional areas of 105–840 nuclei were measured and averaged. Error bars are s.d. *P<0.05, ***P<0.001 (two-tailed Student's t-test).