MISR2 is expressed in primary EOC cultures. Ovarian cancer cells were examined by immunofluorescence by staining with 12G4 primary antibody and Texas Red secondary antibody (A/B: left panels), or FITC-cytokeratin (A/B: right panels), as described in methods. Representative primary ovarian cancer cells are shown in A, and MISR2 overexpressing Z3 cells (positive controls) are shown in panel B. Stained cells were imaged with an LSM 510 Confocal Microscope (Carl Zeiss Micro Imaging, Thornwood, NY).