Skip to main content
. Author manuscript; available in PMC: 2016 Mar 30.
Published in final edited form as: Curr Mol Med. 2016;16(3):222–231. doi: 10.2174/1566524016666160225151131

Fig. (4).

Fig. (4)

Candidate T1R (ALK 2, 3, and 6) are expressed in the primary EOC. Primary cell cultures were analyzed for mRNA (A) and protein expression (B) of ALK 2, ALK 3, ALK 6, and MISR2. (A) Lane 1–4 represents differential mRNA expression of ALK 2, ALK 3, ALK 6 and MISR2 in primary cell cultures detected using RT-PCR. (B) Immunoblotting of primary EOC for MISR2 and T1R expression. Primary EOC lysates were separated on 10% SDS-PAGE under non-reducing conditions, transferred to PVDF membranes and probed with MISR2 or T1R specific Abs. Lane 1–6 demonstrates variable protein expression of T1R and MISR2. GAPDH was used as a control in both RT-PCR and western blotting.