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. Author manuscript; available in PMC: 2016 Mar 30.
Published in final edited form as: Curr Mol Med. 2016;16(3):222–231. doi: 10.2174/1566524016666160225151131

Fig. (5).

Fig. (5)

MIS ligand activates Smad phosphorylation 1/5/8 in MISR2 overexpressing Z3 and primary cell cultures. (A) Z3 cells were untreated or treated with 15ug/ml of MIS or 25ng/ml of BMP4 for 4 hours. Samples were analyzed by western blot analysis for pSmad1/5/8 protein levels in the control, MIS and BMP4-treated groups. (B) Western blot analysis for pSmad1/5/8 protein levels in primary cell cultures. Serum deprived primary cell cultures were treated with diluent or MIS 15ug/ml (upper row) and diluent or 25ng/ml of BMP4 (lower row) for 4 hours respectively and analyzed by immunoblotting as described in methods.