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. Author manuscript; available in PMC: 2016 Mar 30.
Published in final edited form as: Curr Mol Med. 2016;16(3):222–231. doi: 10.2174/1566524016666160225151131

Fig. (6).

Fig. (6)

Effect of MIS on cell viability. (A) MIS induced a dose dependent decrease in cell viability in MISR2 overexpressing Z3 and primary cell cultures whereas no effect was observed in MISR2 negative SKOV3 cell line. Z3, SKOV3 and a representative primary cell culture cells were treated with various concentrations of MIS ranging 3.75–30ug/ml and analyzed using MTT assay for cell viability. (B) Exposure to MIS (30 ug/ml) resulted in decreased cell viability in primary cell cultures. Primary cell cultures (n=5) were treated with 30ug/ml MIS at 24 h after plating and after 72 h, cell viability was analyzed by MTT assay. Results are presented as percentage of control which was calculated using the equation: ((mean absorbance of treated cells/mean absorbance of control cells) x 100). Data are expressed as mean ± standard deviation (SD) from 3 independent experiments.